Learn More About Scientific Equipment & Common Laboratory Questions

01/31/2022

What is DNA Ligase? Definition & Role

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by Excedr /Consumables

What is DNA Ligase? A Brief History DNA ligase is an enzyme that joins DNA strands through the formation of phosphodiester bonds in a process called DNA ligation. The bond in double-stranded DNA is formed by joining the 5′ phosphate and 3′ hydroxyl termini of DNA strands, using ATP as a coenzyme. The discovery and…

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01/31/2022

Featured Manufacturer: Unchained Labs

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by Excedr /Manufacturer

Break Free With Unchained Labs Source Innovation in research labs is highly dependent on technologically advanced instruments and fast processing. Cutting-edge instruments help in reducing errors during experimentation and obtaining increased data volume. They also save time involved in performing the labor-intensive tasks manually. Unchained Labs is just one provider of high-throughput life science biologics…

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01/28/2022

PCR Master Mix: Definition, Types, and More

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by Excedr /Consumables

What is a Real-time PCR Master Mix? PCR master mix, as the name suggests, is a premixed solution of all reagents and essential components required to run a PCR assay. It contains dNTPs (dATP, dGTP, dTTP, and dCTP), Taq DNA polymerase enzymes, MgCl2, stabilizers, and enhancers in a reaction buffer. The PCR system has application…

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01/28/2022

What is the Role of MgCl2 in PCR?

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by Excedr /Consumables

Polymerase Chain Reaction (PCR) Basics Did you know Mullis and Michael Smith won the Nobel Prize for discovering the Polymerase Chain Reaction (PCR) in 1983? This groundbreaking technology-enabled scientists to multiply a single strand of DNA hundreds of billions of times in just a few hours. All that was needed for this experiment was just…

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01/24/2022

Featured Manufacturer: Labconco

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by Excedr /Manufacturer

A Brief History of Labconco It all started in a garage in Kansas. In 1925, Ralph Callaway, a salesman, and Philip Goldfisch, a garage owner, began their partnership and started a laboratory construction company together. Today, that company is known as Labconco Corporation. Labconco is a mid-sized, privately-held laboratory equipment company on a mission to…

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